Phic31 attp site

Author: jznc

August undefined, 2024

WebThe phiC31 (ΦC31) integrase system allows for site-specific integration of transgenes. Further, many attP sites have been characterized for position effects. The bacteriophage phiC31 integrase is a recombinase that mediates the sequence-specific recombination between two attachment sites, attB and attP. Webthrough recombination at sites with sequence similarity to the native attP site (termed pseudo-attP sites). phiC31 integrase can integrate a plasmid of any size, as a single copy, and ... attB site phiC31 integrase donor vector formats SV40 promoter SV40 poly-A CMV TK poly-A pUC ori pFC-CMV-MCS-SV40-Kan/Neo Single Promoter phiC31 Vector Cat ...

phiC31 Integrase Vector System

WebIt is known that phiC31 integrase has the capacity to mediate recombination between the target sequences attP and attB. To test the availability of site-specific integration in the … Web1. dec 2024 · Three novel PhiC31 pseudo-attP sites were identified on chromosomes 1, 3 and 6, and their genomic features were analyzed. The characterized platform cell lines are stable, and because of single-copy, site-specific R4 recognition attP site, the cell lines could be retargeted for recombinant protein production and drug discovery applications. city of sarasota water and sewer

Why PhiC31? - FlyC31

Web8. okt 2024 · An engineered phiC31 “Disintegrase” able to make an attP site in Drosophila out of an attR - attL pair is described. This was used to generate attP sites at genomic locations where a mini- white (mini- w) transgene was subject to … Web8. okt 2024 · One is a mutagenized phiC31 Integrase protein capable of mediating recombination between attR and attL sites to yield an attP site. The other is a P-element … Web21. mar 2012 · The mycobacteriophage large serine recombinase Bxb1 catalyzes site-specific recombination between its corresponding attP and attB recognition sites. Previously, we and others have shown that Bxb1 has catalytic activity in various eukaryotic species including Nicotiana tabacum, Schizosaccharomyces pombe, insects and … city of saratoga encroachment permit

Efficient reversal of phiC31 integrase recombination in ... - PubMed

Pseudo attP sites in favor of transgene integration and

WebExpression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism Depositor Norbert Perrimon Web8. okt 2024 · An engineered phiC31 "Disintegrase" able to make an attP site in Drosophila out of an attR-attL pair is described. This was used to generate attP sites at genomic … city of sarasota water billWeb8. máj 2024 · Recent advances utilizing attP/PhiC31 integrase system has permitted the creation of Drosophila cells in which recombination mediated cassette exchange (RMCE) can be utilized to generate stably integrated transgenic cell lines that contain a single copy of the transgene at the desired locus. city of sarasota roof affidavit

"WebThe present invention provides a method for producing single stranded DNA polynucleotides. In particular, the invention provides a method that utilises a DNA minicircle obtained from a parental minicircle plasmid as a template in an enzyme-mediated rolling circle amplification (RCA) reaction to generate a product that can be cleaved to provide … " - Phic31 attp site

Phic31 attp site

Using a phiC31 “Disintegrase” to make new attP sites in the

Web8. sep 2013 · Phage PhiC31 integrase integrates attB-containing plasmid into pseudo attP site in eukaryotic genomes in a unidirectional site-specific manner and maintains robust … Web17. mar 2006 · The phiC31 integrase catalyzes precise, unidirectional recombination between its 30-40-bp attP and attB recognition sites. In mammalian cells, the enzyme …

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WebThe phiC31 integrase catalyzes unidirectional recombination between two attachment sites, attP and attB, leading to the formation of attL and attR sites . A circular construct harboring an attB site can be efficiently and specifically integrated into … WebSequences of PhiC31 and BxB1 attachment sites For each recombinase, the attB and attP attachment sites share a common central dinucleotide sequence (in red) that is used for …

Web1. aug 2014 · Introduction. I n nature, the phage φC31 integrase recombines an attP site in the phage genome with a chromosomal attB site of its Streptomyces host (Andreas et al., 2002).Mammalian genomes were found to contain “pseudo” attP sites, which have partial sequence identity to the phage attP site and can also mediate efficient integrase activity … WebHere we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane …

Webthrough recombination at sites with sequence similarity to the native attP site (termed pseudo-attP sites). phiC31 integrase can integrate a plasmid of any size, as a single copy, … Web20. aug 2024 · In this study, site-specific integration of a reporter cassette in two PhiC31 pseudo attP sites of CHO cells was done through the homology-directed repair (HDR) generated by the CRISPR/Cas9 system. Altogether, our results demonstrate the possibility of candidate PhiC31 pseudo attP sites as the target sites for targeted integration of a ...

Web12. jún 2024 · The phage-derived phiC31 integrase is a useful tool for mediating sequence-specific genomic integration in mammalian cells, recombining donor plasmids bearing the attB recognition site with introduced genomic attP sites or endogeneous pseudo- attP sites having partial identity to attP.

Web2. jan 2024 · Specifically, the phiC31 integrase recognizes the attP sequence and introduces transgenes into this target site (Thorpe and Smith 1998 ). attP was introduced into the D. … do snakes help the environmentWebphiC31 integrase is a site-specific recombinase from a bacteriophage that has become a useful tool in mammalian cells. The enzyme normally performs precise, unidirectional … city of saratoga springs ny boil waterWebMoreover, (phi)C31 int/attP is used widely as an essential component of integration vectors (such as pSET152) employed in the genetic analysis of Streptomyces species. phiC31 or … city of saratoga springs fire departmentWeb1. jún 2006 · Site-specific integration via ϕC31-mediated RMCE. (A) The target mini-white cassette is flanked by inverted attP sites within a genomic P element, and the donor yellow cassette is flanked by inverted attB sites on a plasmid. Thick solid line, transcription unit; shaded rectangles, P-element ends (not to scale); open triangles, att sites (not to scale). city of saratoga etrakitWeb2. okt 2024 · One possibility is to use the integrase of phage phiC31 (phiC31-int) which catalyzes efficient site-specific recombination between the attP site in the phage genome and the chromosomal attB site of its Streptomyces host. Previous studies showed that phiC31-int is active in many eukaryotic cells, such as murine or human cells, and directs … city of sarasota water deptWeb15. feb 2024 · The attB site (5'-TGCGGGTGCCAGGGCGTGCCCttGGGCTCCCCGGGCGCGTACTCC-3') is one of the two target sites of the phiC31:int integrase. phiC31:int mediates recombination between two non-identical target sites, attP and attB, each of which contains a short integration core … city of sarasota water and sewageWeb23. feb 2010 · The phiC31- att system is derived from the broad host range Streptomyces temperate phage phiC31 [ 11 ]. The 613 amino acid phiC31 protein acts on recognition … do snakes have thermal vision