Pentrd-topo
WebpENTR™/D-TOPO™クローニングキットは、高効率の5分間のクローニング戦略（「TOPO™クローニング」）を利用して、平滑末端PCR産物のベクターへの方向性クロー … WebThe pENTR™/D-TOPO™ Cloning Kits utilize a highly efficient, 5-minute cloning strategy (“TOPO™ Cloning”) to directionally clone a blunt-end PCR product into a vector for entry …
Pentrd-topo
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WebDocuments. The pENTR™/D-TOPO™ Cloning Kits utilize a highly efficient, 5-minute cloning strategy (“TOPO™ Cloning”) to directionally clone a blunt-end PCR product into a vector … Web5. apr 2024 · A recombination reaction was conducted using the Gateway LR Clonase Enzyme Mix (Invitrogen, Carlsbad, CA), TtPK1-pENTRD-topo entry vector, and the pBD destination vector. The resulting TtPK1 “bait” vector in pXDGATcy86 (a derivative of pMC86) was confirmed by sequence analysis to be the GAL4 DNA-binding domain fused in-frame …
WebA repository of over 200,000 plasmids including Protein Structure Initiative protein expression plasmids and vectors, over 75,000 human plasmids, and whole genome collections from many organisms. WebThermo Scientific™ Ultimate™ ORF 克隆集中包含插入序列经过全长测序的高质量人类和小鼠开放阅读框，这些阅读框已克隆到pENTR 221 Gateway入门载体中，可用于各种下游分 …
Web24. nov 2015 · Sensor Kinases RetS and LadS Regulate Pseudomonas syringae Type VI Secretion and Virulence Factors Web12. okt 2024 · Coding sequences of FD and TFL1 were cloned into the pENTRD-TOPO vector. After sequencing, constructs were shuffled into either pDEST32 or pDEST22 (Takara) by …
Web1. máj 2014 · DNA fragments containing NtDRM MTase and NtDRM MTase-M5 were amplified by PCR and were cloned into pENTRD_TOPO vector (Invitrogen) to create pENTRD_NtDRM and pENTRD_NtDRM-M5. These constructs were recombined into the binary vectors pEarleyGate202 and pEarleyGate201 (Earley et al., 2006) to create FLAG …
Webwas amplified by PCR from rPLD1b (26) and subcloned into pENTRd-TOPO (Invitrogen) in frame with an N-terminal His 6 /maltose-binding domain (malE) fusion protein amplified from pSV282 (provided by Laura Mizoue, Vanderbilt Center for Structural Biology). PLD1b.d311 was transferred to pDEST8 baculovirus expression vectors by homolo- dc character godWeb9. júl 2004 · Dulbecco's modified Eagle's medium, fetal bovine serum (FBS), pENTRD/Topo vector, and Gateway cassette were obtained from Invitrogen. Cell Culture—Rat cultured VSMC were prepared as reported before (16, 17). The cells were grown with Dulbecco's modified Eagle's medium with 10% FBS and penicillin/streptomycin and quiescent with … dc characteristics of a mos transistorWebI've followed all recomendations that I have seen here, but I can't get my insert cloned in the pENTR/D-Topo vector. Topo Size 2580 bp. Insert 1038 bp. Topo+Insert 3618 bp. I use 4 ul … geelong chiropracticWebCut sites of enzymes that you select are highlighted to help guide your work. (Enzymes with compatible ends turn the same color.) Selecting cut sites and copying the sequence will … dc character fireflyWebSt-Raymond Veneer Inc., part of The Penrod Group and an integrated manufacturer of high-utility, high-quality face veneer and sliced veneer for the hardwood plywood industry, is … geelong children\u0027s clinic epworthWebGateway®エントリークローン. エントリークローンには、対象遺伝子の両端にattL配列が配置されており、attR配列と組み換えることにより、目的の発現クローンを作成できます … geelong chiro clinicWeb6. okt 2024 · The amplicon was inserted into the entry clone using the pENTR/D-TOPO cloning kit (Invitrogen) in order to yield the StrepII-STOP1WT-pENTRD/topo plasmid. Using this plasmid as a matrix and the primers HA-STOP1-fwd and Ha-STOP1-rev, we PCR amplified StrepII-HA-STOP1. dc character like wolverine