Flp-intm t-rextm 293 细胞系

WebFigure 4-2. TUG-891 but not TUG-1197 displays low potency mediated activation of hFFA1. Flp-InTM T-RExTM 293 cells able to express hFFA1-mVenus-HA were seeded (75,000 cells /well) into poly-D-lysine coated black clear-bottom 96-well plates. Expression of hFFA1 was induced by the antibiotic doxycycline (100 ng/ml) for 18-24 hours. Cells were … WebSep 29, 2024 · 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性（ecotropic）报告质粒和MAPPIT (mammalian protein-protein interaction trap) 报告质粒获得，常用于蛋白互作筛选。 293S 细胞. 293S细胞支持悬浮培养，S为Suspension悬浮的首字母，可以耐受低Ca2⁺培养体系。

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WebBioz Stars score, Techniques, Protocol Conditions and more for Flp Intmt Rextm 293 Cells Hek 293t Hek Human Embryonic Kidney 293 Cells, supplied by Thermo Fisher. Data for … WebFlp-In ™ 293 T-REx 细胞系设计用于快速生成稳定细胞系，确保通过 Flp-In™ 表达载体对您的目标蛋白质进行均一表达。这些细胞在转录活性基因组基因座处包含一个稳定整合的 … green bay packers moccasin slippers https://designchristelle.com

FLPINTREX293_价格-品牌-详情介绍_丁香通 - biomart.cn

WebAbstract. Each subtype of the muscarinic receptor family of G protein-coupled receptors is activated by similar concentrations of the neurotransmitter acetylcholine or closely rel WebFlp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression … Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebApr 2, 2024 · 基本信息. 商品名称：Epigenetics in Cancer 作者：编者:JUNJIANG FU//SABER IMANI 定价：198 出版社：科学 ISBN号：9787030543769 其他参考信息 （以实物为准）. 出版时间：2024-01-01 印刷时间：2024-01-01 版次：1 印次：1 开本：16开 包装：平装 页数：364 内容提要 green bay packers mugs for sale

FlpIn™系统FlpI_价格-品牌-详情介绍_丁香通

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Flp-intm t-rextm 293 细胞系

T-REx™-293 Cell Line - Thermo Fisher Scientific

WebOct 25, 2024 · The Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened ... http://www.bio-thing.com/p118025

Flp-intm t-rextm 293 细胞系

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Web293FTM细胞则是来源于Flp-InTMT-RETM293细胞，该细胞系中转染了ecotropic receptor质粒及MAPPIT(mammalian protein-protein interaction trap)报告质粒，该细胞系主要用于 … WebAug 5, 2024 · 293Flp-InTMT-RExTM Cells. 293Flp-In 293 T-REx细胞系是为了快速生成稳定的细胞系而设计的，它可以确保从Flp-In表达载体上获得的蛋白的均匀表达。这些细胞在转录活跃的基因组位点上包含一个稳定整合的FRT位点，有针对性的整合Flp-In表达载体，确保高水平目的基因。

WebThe Flp-In ™ T‑REx -293 cell line is shipped on dry ice. Store in liquid nitrogen vapor-phase upon receipt. The Flp-In™ T‑REx™-293 cell line is supplied as one vial containing 1 × …

WebT-REx™ 细胞系可稳定表达四环素抑制子蛋白（表 1）。使用 T-REx™ 系统可以节省大量的时间和精力。通过使用阳性对照载体 pcDNA™4⁄TO⁄lacZ 进行瞬时转染，对 T-REx™ 细胞系进行功能检测。在用四环素诱导时，T-REx™ 细胞系在抑制状态下表现出极低的基础表达水平，表达水平较高（图 1）。 WebFeb 20, 2024 · 在我们以前的工作中，我们在四环素诱导型CMV启动子（HEK-293 Flp-In T-REx-Thermo）的控制下，建立了表达C末端3xFLAG标记的外来体成分EXOSC10（RRP6）的同基因HEK-293细胞系费雪科学）。. 该系统允许我们以与内源WT蛋白质相当的水平表达标记的EXOSC10蛋白质，并且使用磁性 ...

WebFigure 5-9. PMA induces internalisation of hFFA4. Flp-InTM T-RExTM 293 cells able to express FLAG-hFFA4-mVenus were plated down onto poly-D-lysine-coated 30 mm glass coverslips and receptor expression was induced using doxycycline (100 ng/ml). The internalisation of hFFA4 in live cells was then imaged using a Zeiss VivaTome spinning …

WebSep 29, 2024 · 293FTM 细胞 是由 Flp-InTM T-RExTM 293 ... 总之， 293系列细胞经过长时间的改造与优化，已在多个领域实现了较多的应用，源井生物对于293与293T细胞也有丰富的基因编辑和细胞模型构建的经验，并拥有上千种293细胞的KO现货，覆盖超20种信号通 … flower shops in doverWebFlp-In™ stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In™ system (Flp-In™ T-Rex™) allows … green bay packers most famous coachWebgenomic location. To generate these cell lines, the Flp-In ™ T-REx System involves the following major steps: 1. Independent integration of the following two plasmids into the … green bay packers mug ukWebApr 21, 2024 · Flp-In 293 T-REx细胞系是为了快速生成稳定的细胞系而设计的，它可以确保从Flp-In表达载体上获得的蛋白的均匀表达。 这些细胞在转录活跃的基因组位点上包含 … green bay packers motorcycle accessoriesWebFigure 3-20. Relative expression of FLAG-hFFA4-mVenus and FLAG-hFFA4-GFP2 in a Flp-InTM T-RExTM 293 cell line. Flp-InTM T-RExTM 293 double stable cells able to express inducibly FLAG-hFFA4-mVenus and constitutively FLAG-hFFA4-GFP2 were seeded into poly-Dlysine coated black 96-well plates. Cells were treated with varying concentrations … green bay packers mouthguardWebHuman. The Flp-In T-REx-293 cell line stably expresses the lacZ-Zeocin fusion gene and the Tet repressor, and is designed for use with the Flp-In T-REx System. The cell line expresses the Tet repressor from pcDNA6/TR and contains a single integrated Flp Recombination Target (FRT) site from pFRT/lacZeo as confirmed by Southern blot analysis. flower shops in dornochWebOct 31, 2024 · HEK293细胞及其衍生株HEK293（human embryonic kidney 293），简称293细胞，来源于人胚胎肾细胞，由于在19号染色体整合了大约4.35 kb的腺病毒基因组片段，使其细胞周期以及细胞表型、核型等发生了 ... 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性（ecotropic）报告质粒和 ... green bay packers music video