Brdu antibody rat
WebDec 18, 2012 · The images in the inserts were acquired for the same time (190 ms for cells labelled with anti-rat antibody FITC conjugate and 74 ms for cells labelled with anti-rat Cy3 conjugate). Their intensity therefore reflects the decrease of the EdU-derived signal of the anti-BrdU antibody signal after the click reaction with respect to the control cells. WebFeb 19, 2008 · The cells were then incubated in 1.5 M HCl at room temperature for 1 h with gentle rocking (to denature the DNA and expose the BrdU epitope), followed by standard immunodetection of BrdU by using a mouse anti-BrdU monoclonal antibody (clone BU-33, obtained from Sigma) and an Alexa488-conjugated anti-mouse secondary antibody …
Brdu antibody rat
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WebDescription. Bromo-deoxyuridine (BrdU) is a thymidine analog that is used in cell proliferation studies. BrdU in culture is incorporated into the DNA during DNA synthesis. Cellular incorporation of BrdU can be detected by anti-BrdU specific antibodies following membrane permeabilization by flow cytometry or immunohistochemistry. WebPrecisionAb Rat Anti-BrdU Antibody, clone RF04-2 The Rat Anti-BrdU Antibody, clone RF04-2 has been tested in immunohistochemistry and flow cytometry. The antibody …
WebAntibodies that detect BrdU can be used in several scientific applications, including Immunohistochemistry, Immunocytochemistry, Flow Cytometry, ELISA and Western Blot. … WebTarget Information. Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be …
WebThe monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Recommended Assay Procedures This product is NOT recommended to use with BrdU flow kits, e.g. Cat. No. 559619. Please see the detailed protocol under "Cell Cycle/DNA Analysis" at our website: www.bdbiosciences.com/resources/index.jsp WebBromodeoxyuridine/BrdU Alexa Fluor® 488-conjugated Antibody Cat # IC7225G Citations (1) Human/Mouse Myogenin Antibody Cat # MAB6686 Citations (1) 5-BrdU Cat # 5015 (1) Human IQGAP2 Antibody Cat # AF5068 Human FGFR3 (IIIb) Antibody Cat # MAB1474 Citations (1) Human Alkaline Phosphatase/ALPL Alexa Fluor® 647-conjugated Antibody …
Webab152095 detects BrdU protein from HeLa treated with 10 μM BrdU for 30 minutes and fixed in 4% paraformaldehyde at 4ºC for 15 minutes by flow cytometry analysis. Black: …
WebLatrophilin-2 is a novel receptor of LRG1 that rescues vascular and neurological abnormalities and restores diabetic erectile function. View PDF. In Experimental & Molecular Medicine on 1 May 2024 by Yin, G. N., Kim, D. K., et al.. codes wilayaWebBrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for … code swift royal bank of canadaWebSpecificity. This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or … code swift orabank senegalWebTarget Information. Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of … codes wiki ro ghoulWeb5′-Bromo-2-deoxyuridine (BrdU) is a carcinogen that may be absorbed by skin or inhalation. BrdU solutions are sensitive to light. [ 1] Monoclonal anti-BrdU (immunohistology grade) … cals cartsWebBromodeoxyuridine (BrdU) is a uridine derivative that can be incorporated specifically into DNA in place of thymidine. Anti-BrdU identifies BrdU (but not thymidine) in single … code swift ou bicWebThe BD Pharmingen™ BrdU In-Situ Detection Kit is designed for immunohistochemical staining of BrdU in frozen sections, formalin-fixed paraffin-embedded sections, and cultured or isolated cells on slides. Our monoclonal antibody against BrdU works in all species tested (human, mouse, and rat) and provides improved specific staining with minimal … calscape blue eyed grass